Hemocytometer Cell Count
Calculate cell concentration, total cells and viability from a hemocytometer count, free online.
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Each large square of a Neubauer chamber holds 0.1 µL (1 mm² × 0.1 mm depth), so cells/mL = (cells counted ÷ squares counted) × dilution factor × 10⁴. With trypan blue staining, viability = live ÷ (live + dead).