Hemocytometer Cell Count

Calculate cell concentration, total cells and viability from a hemocytometer count, free online.

Live (unstained) cells counted

Dead (stained) cells counted (optional)

Large squares counted

Dilution factor (e.g. 2 for 1:1 with dye)

Total suspension volume (mL)

About this calculator

Each large square of a Neubauer chamber holds 0.1 µL (1 mm² × 0.1 mm depth), so cells/mL = (cells counted ÷ squares counted) × dilution factor × 10⁴. With trypan blue staining, viability = live ÷ (live + dead).